My Summer Research Project is about the Structure and Function of a protein called JIL-1 and its Interaction with lamin. Lamin is a component of the nucleus of the cell that gives its structure and support. I’m working with two projects. In the first project we want to determine the structure or part of the protein. In the second project we want to test what does the protein do to the component of the nucleus in S2 cells.
In the first project, the protein will be examined by a technique that requires a large amount of quantity so I’ll be producing and purificating protein. In the second project I repeat Xiaomin’s project who determined their interaction with two kinds of fruit flies: the wild-type, that has the protein, and the mutated, that doesn’t have the protein. She observed that in a technique with electric charge the one that has the protein travel slower, because it is heavier, than the one that doesn’t. What I’m trying to test is if it does the same using the protein extract from the S2 cells, but instead of mutation we use another technique to remove the protein. This can provide a vision in how a structure of the nucleus can be regulated by the protein.
